As of the August 2026 package, it can - as a choice, not a default. The chatbot and its widget surface are two settings, both off until you enable them. Switch both on and visitors who arrive after hours get the AI assistant instead of only the message form, grounded in your knowledge base, with a per-visitor answer budget - and anything the bot can't resolve still lands in the missed-chat queue your morning digest covers.
Worth weighing before you do: overnight is when questions are least likely to be routine and a wrong answer sits unchallenged for eight hours. Some teams genuinely want AI there; others find that capturing the message well and replying first thing beats a bot guessing at 3am. We wrote up when not to use a chatbot for exactly this decision.